Profilbillede af amittambvekar
Flag for India Bangalore, India
Medlem siden 19. marts 2012
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Over the 3 years, Assisting people in Molecular biology, genetics, Nutraceutical, Pharmaceutical and food businesses and companies.I am working as a professional freelancer My core competency lies in complete end-end management. I am seeking opportunities to go into partnership with excellent employers for a long term
$5 USD/hr
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Research Scientist

Apr 2008

Projects undertaken:\no"Cloning and expression of human Glucokinase regulatory protein gene (GCKR) in E. coli" at IBAB, \no"'Studies on antioxidant and antimicrobial potential of pigments of Serratia, Pseudomonas, Micrococcus and Staphylococcus species-\n\nSkills:\noAll basic molecular biology techniques (Electrophoresis, SDS PAGE, Blotting techniques, PCR, DNA- RNA-Protein isolation, Primer designing, cloning etc)\noCytotoxic assays (MTT, LDH, Neutral red & Trypan blue



2005 - 2007 (2 years)


PGDB (2009)




"Cloning and expression of human Glucokinase regulatory protein gene (GCKR) in E. coli" at IBAB

Project [login to view URL], IBAB, Dec 2009-June-2010(7-months)-Work includes\n? Identification of tissue where GCKR expressed using bioinformatics tools\n? Ordering cell-lines, culturing, reviving, passaging (HepG2)\n? Cloning- total RNA extraction, c-DNA preparation, PCR amplification\n? Designing of specific primers for PCR amplification of specific c-DNA\n? Cutting plasmid with restriction enzymes, and annealing of cloned gene\n? Transformation and identification of cloned cells, preservation\n? expressi


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