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Research AssociateNov 2010
Working with a team of researchers on projects associated with microRNA, extra-cellular vesicles, and cancer diagnostics. Responsible for computer research, professional and technical writing, proofreading, data entry, statistical analysis, and figure formatting/creation. Perform standard molecular biology techniques such as qPCR, western blotting, and protein/nucleic acid extraction and purification daily.
BS - Molecular Biology2005 - 2009 (4 years)
Employee of the Month (2011)Panera Bread, LLC
Awarded employee of the month for my outstanding customer service and dedication to the job.
Presidential Scholar Candidate (2005)
I was a Presidential Scholar Candidate in 2005 as a result of my very high ACT scores.
Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
Circulating microRNAs (miRNAs) have emerged as candidate biomarkers of various diseases and conditions including malignancy and pregnancy. This approach requires sensitive and accurate quantitation of miRNA concentrations in body fluids. Herein we report that enzyme-based miRNA quantitation, which is currently the mainstream approach for identifying differences in miRNA abundance among samples, is skewed by endogenous serum factors that co-purify with miRNAs and anticoagulant agents used during collection.
Sensitive PCR-based quantitation of cell-free circulating microRNAs.
Cell-free microRNAs (miRNAs) that circulate in the blood are promising surrogate biomarkers of disease and physiological processes. The ease of quantifying specific miRNA species using made-to-order approaches based on Taq-polymerase has led to numerous studies that have identified changes in the abundance of circulating cell-free miRNA species that correlate with pathology or other events. The growing interest in developing miRNAs as blood biomarkers necessitates the careful consideration of the unique pro
MicroRNAs are exported from malignant cells in customized particles.
MicroRNAs (miRNAs) are released from cells in association with proteins or microvesicles. We previously reported that malignant transformation changes the assortment of released miRNAs by affecting whether a particular miRNA species is released or retained by the cell. How this selectivity occurs is unclear. Here we report that selectively exported miRNAs, whose release is increased in malignant cells, are packaged in structures that are different from those that carry neutrally released miRNAs (n-miRNAs),
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