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Polish the English language of a scientific manuscript Ferulic acid (FA) in H9C2 cells - 30/04/2018 04:22 EDT

The study deals with Ferulic acid (FA). We investigate its protective effect on H/R injury in H9C2 cells by inhibiting mitochondrial hyperautophagy through the PINK1/parkin pathway, protecting mitochondrial function, and inhibiting apoptosis. First, FA was detected to reduce apoptosis (CCK8 detection survival rate, TUNEL detection of apoptosis, Caspase3 protein WB expression), and secondly, increased mitochondrial function (ADP/ATP conversion rate decreased, membrane potential increased, ROS decreased) Finally, the mechanism was explored from the PINK1/pakin pathway. The mitochondrial autophagy agonist rapamycin was added and it was found that FA can antagonize the activation of rapamycin on this pathway and reduce mitochondrial autophagy. (Fluorescence probe colocalization was used to detect mitochondrial-lysosome binding levels; immunofluorescence co-localization was used to detect PINK1-HSP60, Parkin-HSP60 levels, which is the expression level of the pathway proteins on mitochondria; WB assays PINK1, Parkin, LC3II/LC3I, P62 protein expression level)

Evner: Engelsk Grammatik, Videnskabelig Forskning, Teknisk Forfatning

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( 2 bedømmelser ) Utrecht, Netherlands

Projekt ID: #16831702

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