Thesis writing Botany: TEST SYSTEM & TREATMENT SCHEDULE

TEST SYSTEM & TREATMENT SCHEDULE: Allium cepa –Taken as a model plant, Bulbs were germinated in sterilized moist sand, Seedlings then transferred to the hydroponic medium.

PREPARATION OF GROWING MEDIUM

· Half strength modified Hoagland Medium selected for hydroponics

· Control - Normal Hoagland Medium

· Experiment 1- Iron Chelate in nutrient medium replaced with Iron oxide Nanoparticle

· Experiment II - Copper sulfate in nutrient medium replaced with Copper oxide Nanoparticle

Various concentrations (mM) of Iron Oxide and Copper Oxide NPs in suspension form prepared in deionized water- NPs suspended in deionized water-Dispersed using magnetic stirrer-Ultrasonicated for 30 min.

Approved objectives of research topic:

· To investigate the impact of metal oxide NPs in the vegetative system.

· To analyze phytotoxicity of NPs for cytogenetic abnormalities in the roots.

· To determine the penetration and translocation of NPs in organelles and tissues by TEM images of vegetative parts.

· To determine the uptake of elements in the xylem sap and control by ICP-MS and ICP-AES.

· To determine the oxidative damage to [login to view URL] tissues after intake of nanoformulation

· To find the disease resistance potential of NP exposed [login to view URL] inoculated with Fusarium oxysporum.

Evner: Research Writing

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Projekt ID: #28993360