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We are Grade 12 STEM students from Bataan National High School–Senior High School conducting a research study entitled “Effects of Extraction Method and Extract Concentration on the Antifungal Activity of Dracaena trifasciata ‘Laurentii’ Leaf Extract Against Saccharomyces cerevisiae.” Our study aims to determine whether the extraction method (aqueous and ethanolic) and extract concentration (25%, 50%, 75%, and 100%) affect the antifungal activity of snake plant leaf extract against Saccharomyces cerevisiae. The plant leaves will undergo preparation and extraction, while the resulting extracts will be evaluated through an appropriate laboratory antifungal susceptibility assay. We are seeking the assistance of a qualified microbiologist to supervise and provide technical guidance during the microbiological portion of the study. Specifically, we need assistance in verifying the suitability of the S. cerevisiae culture, preparing and standardizing the fungal inoculum, selecting the appropriate culture medium and antifungal testing procedure, establishing suitable controls, ensuring proper aseptic technique and laboratory safety, and interpreting the resulting inhibition data. All microbiological procedures involving the live culture will be conducted under the supervision of the microbiologist and according to the partner laboratory's established protocols. The expected output of the project is a comparative evaluation of the antifungal activity of the different snake plant extracts based on their measured zones of inhibition, allowing us to determine whether extraction method and concentration influence the observed antifungal activity. Assistance We Are Looking For Technical supervision of the microbiological procedures Verification of Saccharomyces cerevisiae culture Guidance on appropriate fungal culture medium and assay Preparation/standardization of the fungal inoculum Guidance on appropriate positive and negative controls Supervision of the antifungal assay Proper measurement and recording of inhibition zones Guidance on laboratory safety, aseptic technique, and waste disposal Assistance in interpreting the microbiological results
Project ID: 40675982
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9 freelancers are bidding on average $42 USD for this job

Welcome! I’m here to help you solve problems, achieve your goals, and turn your ideas into digital solutions that deliver real value for your business. I understand your research study on the antifungal activity of Dracaena trifasciata ‘Laurentii’ leaf extract against Saccharomyces cerevisiae is crucial for your academic path. With my background in microbiology, I can provide the technical supervision you need for your microbiological procedures. From verifying the suitability of your S. cerevisiae culture to guiding you on preparation and standardization of the fungal inoculum, I will ensure that your laboratory work adheres to established protocols. I can also assist with selecting the right culture medium, establishing controls, and interpreting your results effectively. From our first conversation to final delivery, you can expect clear communication, reliable service, and a friendly, professional approach. Your goals come first. Let’s build something that moves your research forward.
$40 USD in 7 days
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Hi, I’m Maximillian, a Full Stack Developer specializing in modern websites and digital experiences that help businesses build credibility, communicate their value, and connect with their customers. I came across your project and would love the opportunity to help bring your vision to life. I don't see this as simply providing guidance for a research study. I see an opportunity to support your academic journey and contribute to valuable scientific exploration. Your research on the antifungal activity of Dracaena trifasciata ‘Laurentii’ leaf extract is not just an academic exercise; it has real-world implications for understanding natural antifungals. I can offer the expertise you need to ensure your microbiological procedures are executed with precision and care. What I Can Bring to Your Project ✓ Technical supervision of microbiological procedures ✓ Verification of Saccharomyces cerevisiae culture ✓ Guidance on culture medium and antifungal assay ✓ Support in preparing and standardizing fungal inoculum ✓ Advice on appropriate controls and safety protocols ✓ Assistance in measuring and recording inhibition zones ✓ Expertise in interpreting microbiological results My Approach I believe successful research starts with understanding the goals and challenges at hand. I will take the time to grasp your objectives, ensuring that my guidance is tailored to meet your specific needs. If we work together, you’ll benefit from my attention to detail, commitment to scientific integrity, and a collaborative spirit. I look forward to supporting your study and contributing to your findings. Regards, Maximillian
$40 USD in 7 days
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Hello, I have carefully reviewed your research project on the antifungal activity of Dracaena trifasciata ‘Laurentii’ leaf extracts against Saccharomyces cerevisiae. I understand that you need qualified microbiological guidance for the study, including verification of the fungal culture, appropriate assay selection, preparation and standardization of the inoculum, suitable controls, proper measurement of inhibition zones, and interpretation of the resulting data. I can provide organized technical support while following the established protocols of your partner laboratory and maintaining appropriate laboratory safety and documentation. I can also help with accurate recording and comparative analysis of the inhibition-zone results for the different extraction methods and concentrations. My focus would be on accuracy, reproducibility, clear documentation, and scientifically sound interpretation of the results. I am also comfortable communicating technical concepts clearly so that the research team can understand each stage of the study. I would be happy to discuss your existing protocol, expected deliverables, and timeline before beginning. Thank you for considering my proposal.
$35 USD in 7 days
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Hi, I’d be happy to help you with this project. I have strong attention to detail and experience working with data, research, and accuracy-focused tasks. I understand the importance of delivering clean, reliable, and well-organized work within the required deadline. I will carefully follow your requirements, communicate clearly throughout the project, and make sure the final work meets your expectations. I’m available to start immediately and can complete the project within the specified timeframe. I’d be glad to discuss the details and get started. Thank you for considering my proposal.
$45 USD in 7 days
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Hello, I am owner of a professional diploma in Biological Sciences. Have proven experience in experimental design including statistical analyses (check my portafolio). I have used softwares like RStudio, Past or Statistix but feel comfortable to tell me your preferences. Otherwise, I can give you suggestions for experimental design (number of replicates, lab protocols) and further (statistics, graph design). Feel free to contact me, Greetings. Juan Martín-Lesmes
$35 USD in 6 days
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Hello, I'm a medical biochemistry and molecular biology graduate with a Web of Science researcher profile and hands-on microbiology laboratory experience. I understand both the technical rigor your study needs and the realities of working within a partner laboratory's protocols and safety requirements as Grade 12 STEM researchers. For the microbiological portion of your antifungal activity study, I can provide: • Culture verification: confirming the suitability and viability of your Saccharomyces cerevisiae strain before testing begins • Inoculum preparation: standardizing fungal inoculum density to ensure reliable, reproducible results across your extraction method and concentration groups • Assay selection and setup: guidance on the most appropriate antifungal susceptibility method (e.g., disc or well diffusion) for comparing aqueous vs. ethanolic extracts at 25–100% concentrations • Controls: establishing scientifically sound positive and negative controls for valid comparison • Aseptic technique and safety: supervising proper sterile procedure, contamination prevention, and safe waste disposal throughout • Data interpretation: helping you accurately measure, record, and interpret zones of inhibition, and translate that into a defensible comparative evaluation I'd welcome the chance to support your research and help you produce results you can stand behind. Happy to discuss your current timeline and lab access.
$45 USD in 7 days
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Greetings! As a microbiologist, I would be glad to supervise and guide your Grade 12 STEM research project on Dracaena trifasciata leaf extracts against Saccharomyces cerevisiae. Here is a structured overview of how I will support your study from start to finish: Technical Supervision & Guidance Plan Culture & Inoculum Setup: Verification of the S. cerevisiae strain, selection of appropriate media (e.g., Sabouraud Dextrose Agar/Broth or YPD), and standardization of the fungal inoculum using a 0.5 McFarland standard or spectrophotometry to ensure consistency across trials. Assay Design & Testing: Recommendation and execution oversight of an appropriate testing procedure (such as Kirby-Bauer agar well diffusion or disc diffusion), ensuring precise setup for the two extraction methods (ethanolic vs. aqueous) across all four concentration levels (25%, 50%, 75%, 100%). Controls & Biosafety: Selection of suitable positive (e.g., standard antifungal like Nystatin or Fluconazole) and negative controls (solvent blanks), enforcing strict aseptic techniques, laboratory biosafety guidelines, and safe biohazard waste disposal. Data Analysis & Result Interpretation: Methodical measurement and recording of the inhibition zones (in mm), followed by technical assistance in interpreting the statistical significance of how extraction methods and concentrations impact antifungal efficacy.
$45 USD in 7 days
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