FlowJo Cytometry Data Analysis

I have a mixed set of single-cell and population flow-cytometry files that I need processed in FlowJo. My immediate goal is to apply a combination of my existing “go-to” gates alongside fresh, on-the-fly adjustments so the final workspace captures every relevant sub-population without bias. The core analysis I’m after is a clear, publication-ready histogram overlay that makes trend comparison effortless. Once the gating is locked, I’d like the full FlowJo workspace returned, accompanied by exported statistics (mean, median, % positive, etc.) and high-resolution plots ready for insertion into figures. You’ll receive the raw .fcs files plus a short note outlining my current gate hierarchy. I’m happy to jump on a quick call or share screenshots so you see exactly where the predefined gates sit and where creative gating could add value. Deliverables • Updated FlowJo workspace (.wsp) with combined gating strategy implemented • Histogram overlay files (PNG or PDF) for all specified markers/parameters • Exported stats tables (CSV or Excel) for downstream analysis Acceptance criteria 1. All provided files open without errors in FlowJo v10. 2. Histogram overlays align correctly between samples and clearly label each population. 3. Stats tables correspond to the final gating tree and match the overlays. If you’re fluent in FlowJo and comfortable juggling both standard and exploratory gates, let’s get started.

Project ID: 40348781